Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PCMT1) Polyclonal Antibody, Unconjugated (ER1914-87) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Hippocampus (Mouse) Lysate at 40 ug HL60(Human) Cell Lysate at 30 ug Primary: Anti- PCMT1 (ER1914-87) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 28 kD| Product Name | PCMT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | L isoaspartyl protein carboxyl methyltransferase L isoaspartyl/D aspartyl methyltransferase PCMT 1 PIMT Protein beta aspartate methyltransferase Protein L isoaspartate (D aspartate) O methyltransferase PIMT_HUMAN Protein L isoaspartyl/D aspartyl methyltransferase. |
| Product description | Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human PCMT1 165-227/227 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | L isoaspartyl protein carboxyl methyltransferase L isoaspartyl/D aspartyl methyltransferase PCMT 1 PIMT Protein beta aspartate methyltransferase Protein L isoaspartate (D aspartate) O methyltransferase PIMT_HUMAN Protein L isoaspartyl/D aspartyl methyltransferase. |
|---|---|
| Molecular Weight(MW) | 25 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P22061 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PCMT1) Polyclonal Antibody, Unconjugated (ER1914-87) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Hippocampus (Mouse) Lysate at 40 ug HL60(Human) Cell Lysate at 30 ug Primary: Anti- PCMT1 (ER1914-87) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 28 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
