Application
Fig1: Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μgL 1X PBS containing 0.5% BSA(green).Application
Fig2: Sample:Brain(Mouse) Lysate at 30 ug Primary: Anti-PDE4D (ER1914-89) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 100 kDApplication
Fig3: Sample:Brain(Mouse) Lysate at 30 ug Primary: Anti-PDE4D (ER1914-89) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 90kDApplication
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- PDE4D (ER1914-89) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90kD Observed band size: 90 kD| Product Name | PDE4D |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | cAMP specific 3',5' cyclic phosphodiesterase 4D DKFZp686M11213 FLJ97311 cAMP specific 3' 5' cyclic phosphodiesterase 4D cAMP specific phosphodiesterase 4D cAMP specific phosphodiesterase PDE4D cAMP specific phosphodiesterase PDE4D6 cyclic AMP phosphodiesterase DPDE 3 DPDE3 Dunce like phosphodiesterase E3 HSPDE4D PDE 43 PDE43 PDE4DN2 Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) Phosphodiesterase 4D cAMP specific Phosphodiesterase 4D, cAMP specific (dunce) Phosphodiesterase E3 dunce homolog Drosophila STRK 1 STRK1. |
| Product description | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. |
| Immunogen | KLH conjugated synthetic peptide derived from human PDE4D 401-550/809 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | cAMP specific 3' 5' cyclic phosphodiesterase 4D DKFZp686M11213 FLJ97311 cAMP specific 3' 5' cyclic phosphodiesterase 4D cAMP specific phosphodiesterase 4D cAMP specific phosphodiesterase PDE4D cAMP specific phosphodiesterase PDE4D6 cyclic AMP phosphodiesterase DPDE 3 DPDE3 Dunce like phosphodiesterase E3 HSPDE4D PDE 43 PDE43 PDE4DN2 Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) Phosphodiesterase 4D cAMP specific Phosphodiesterase 4D cAMP specific (dunce) Phosphodiesterase E3 dunce homolog Drosophila STRK 1 STRK1. |
|---|---|
| Molecular Weight(MW) | 90 KDa |
| Cellular Localization | Cytoplasm. Membrane. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Note=Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome. |
| SwissProt ID | Q08499 |
|---|
Application
Fig1: Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μgL 1X PBS containing 0.5% BSA(green).
Application
Fig2: Sample:Brain(Mouse) Lysate at 30 ug Primary: Anti-PDE4D (ER1914-89) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 100 kD
Application
Fig3: Sample:Brain(Mouse) Lysate at 30 ug Primary: Anti-PDE4D (ER1914-89) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 90kD
Application
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- PDE4D (ER1914-89) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90kD Observed band size: 90 kD| Positive Control | Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
