Application
Fig1: Sample: Brain (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti-PDE7B (ER1914-90) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 52 kD Observed band size: 52 kDApplication
Fig2: Blank control(blue): RSC96 cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- PDE7B antibody(ER1914-90), Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Application
Fig3: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-PDE7B (ER1914-90) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD| Product Name | PDE7B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDE 7B PDE7B protein Phosphodiesterase 7B Phosphodiesterase7B RNPDE7B Rolipram insensitive phosphodiesterase type 7 bA472E5.1 cAMP specific 3 5 cyclic phosphodiesterase 7B cAMP specific 3'5' cyclic phosphodiesterase 7B High affinity cAMP specific 3 5 cyclic phosphodiesterase High affinity cAMP specific 3'5' cyclic phosphodiesterase PDE7B_HUMAN. |
| Product description | PDE7B is a cAMP specific phosphodiesterase. The cAMP-specific phosphodiesterase type-7 (PDE7) family is comprised of 2 genes (PDE7A and PDE7B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. PDE7B has three splice variants (PDE7B1 = 446 amino acid; PDE7B2 =359 amino acids and PDE7B3 = 459 amino acids. Both PDE7B2 ad PDE7B3 posses unique N-terminal sequences. PDE7B is expressed in various tissues including skeletal muscle, heart, lung and testis. PDE7B may be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain. |
| Immunogen | KLH conjugated synthetic peptide derived from human PDE7B 281-360/450 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | PDE 7B PDE7B protein Phosphodiesterase 7B Phosphodiesterase7B RNPDE7B Rolipram insensitive phosphodiesterase type 7 bA472E5.1 cAMP specific 3 5 cyclic phosphodiesterase 7B cAMP specific 3'5' cyclic phosphodiesterase 7B High affinity cAMP specific 3 5 cyclic phosphodiesterase High affinity cAMP specific 3'5' cyclic phosphodiesterase PDE7B_HUMAN. |
|---|---|
| Molecular Weight(MW) | 52 KDa |
| SwissProt ID | Q9NP56 |
|---|
Application
Fig1: Sample: Brain (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti-PDE7B (ER1914-90) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 52 kD Observed band size: 52 kD
Application
Fig2: Blank control(blue): RSC96 cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- PDE7B antibody(ER1914-90), Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Application
Fig3: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-PDE7B (ER1914-90) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD| Positive Control | Highly expressed in brain. Also expressed in heart, liver, skeletal muscle and pancreas. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
