Application
Fig1: Blank control (blue line):Hela(blue). Primary Antibody (green line): Rabbit Anti-PDGF-B antibody (ER1914-92) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Stomach (Rat) Lysate at 40 ug Primary: Anti-PDGF-B (ER1914-92) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 32 kD| Product Name | PDGF-B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDGF beta PDGF-B Platelet-derived growth factor B-chain PDGF AB + BB Becaplermin c sis FLJ1285 Oncogene SIS PDGF 1 PDGF 2 PDGF A PDGF A chain PDGF B chain PDGF subunit A PDGF subunit B PDGF1 PDGF2 PDGFA PDGFB PDGFR1 Platelet derived growth factor 2 Platelet derived growth factor alpha Platelet derived growth factor alpha chain Platelet derived growth factor alpha polypeptide Platelet derived growth factor B chain Platelet derived growth factor beta Platelet derived growth factor beta polypeptide Platelet derived growth factor subunit A Platelet derived growth factor subunit B SIS SSV v sis platelet derived growth factor beta polypeptide. |
| Product description | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. |
| Immunogen | KLH conjugated synthetic peptide derived from human PDGF-B 155-210/241 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatDogCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | PDGF beta
PDGF-B
Platelet-derived growth factor B-chain PDGF AB + BB Becaplermin c sis FLJ1285 Oncogene SIS PDGF 1 PDGF 2 PDGF A PDGF A chain PDGF B chain PDGF subunit A PDGF subunit B PDGF1 PDGF2 PDGFA PDGFB PDGFR1 Platelet derived growth factor 2 Platelet derived growth factor alpha Platelet derived growth factor alpha chain Platelet derived growth factor alpha polypeptide Platelet derived growth factor B chain Platelet derived growth factor beta Platelet derived growth factor beta polypeptide Platelet derived growth factor subunit A Platelet derived growth factor subunit B SIS SSV v sis platelet derived growth factor beta polypeptide. |
|---|---|
| Molecular Weight(MW) | 27 KDa |
| Cellular Localization | Secreted. Note=Released by platelets upon wounding. |
| SwissProt ID | P01127 |
|---|
Application
Fig1: Blank control (blue line):Hela(blue). Primary Antibody (green line): Rabbit Anti-PDGF-B antibody (ER1914-92) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Stomach (Rat) Lysate at 40 ug Primary: Anti-PDGF-B (ER1914-92) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 32 kD| Positive Control | Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
