Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PDZD8 Polyclonal Antibody, Unconjugated (ER1914-94) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-PDZD8 (ER1914-94) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 128 kD Observed band size: 130 kDApplication
Fig3: Sample: Panc-1(Human) Cell Lysate at 30 ug Primary: Anti-PDZD8 (ER1914-94) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 128 kD Observed band size: 130kD| Product Name | PDZD8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDZ domain containing 8 PDZ domain containing protein 8 PDZD 8 PDZK 8 PDZK8 Sarcoma antigen NY SAR 104 Sarcoma antigen NY SAR 84 Sarcoma antigen NY SAR 84/NY SAR 104 bA129M16.2 FLJ25412 FLJ34427 OTTHUMP00000046868 PDZD8_HUMAN. |
| Product description | Plays a role in the regulation of cell morphology and cytoskeletal organization. May inhibit herpes simplex virus 1 infection at an early stage. May promote retroviral infection. |
| Immunogen | KLH conjugated synthetic peptide derived from human PDZD8 901-1000/1154 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogHorse |
| Concentration | 1mg/ml |
| Alternative Names | PDZ domain containing 8 PDZ domain containing protein 8 PDZD 8 PDZK 8 PDZK8 Sarcoma antigen NY SAR 104 Sarcoma antigen NY SAR 84 Sarcoma antigen NY SAR 84/NY SAR 104 bA129M16.2 FLJ25412 FLJ34427 OTTHUMP00000046868 PDZD8_HUMAN. |
|---|---|
| Molecular Weight(MW) | 128 KDa |
| SwissProt ID | Q8NEN9 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PDZD8 Polyclonal Antibody, Unconjugated (ER1914-94) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-PDZD8 (ER1914-94) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 128 kD Observed band size: 130 kD
Application
Fig3: Sample: Panc-1(Human) Cell Lysate at 30 ug Primary: Anti-PDZD8 (ER1914-94) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 128 kD Observed band size: 130kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
