Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- PDZK1 (ER1914-96) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD| Product Name | PDZK1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAP70 CFTR associated protein of 70 kDa CFTR associated protein, 70-KD CFTR-associated protein of 70 kDa CLAMP D3Ertd537e Dietary Pi-regulated RNA-1 Diphor-1 mPDZK1 Na(+)/H(+) exchange regulatory cofactor NHE-RF3 Na(+)/H(+) exchanger regulatory factor 3 Na/Pi cotransporter C-terminal-associated protein 1 Na/Pi cotransporter C-terminal-associated protein NaPi Cap1 NaPi-Cap1 NaPiCap1 NHERF 3 NHERF-3 NHERF3 NHRF3_HUMAN OTTHUMP00000015572 PDZ domain containing 1 PDZ domain containing protein 1 PDZ domain-containing protein 1 PDZD1 PDZK1 Sodium hydrogen exchanger regulatory factor 3 Sodium-hydrogen exchanger regulatory factor 3 1700023D20Rik 2610507N21Rik 4921513F16Rik AI267131 AI314638 AL022680 C terminal linking and modulating protein. |
| Product description | A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human PDZK1 1-100/519 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml |
| Alternative Names | CAP70
CFTR associated protein of 70 kDa
CFTR associated protein 70-KD CFTR-associated protein of 70 kDa CLAMP D3Ertd537e Dietary Pi-regulated RNA-1 Diphor-1 mPDZK1 Na(+)/H(+) exchange regulatory cofactor NHE-RF3 Na(+)/H(+) exchanger regulatory factor 3 Na/Pi cotransporter C-terminal-associated protein 1 Na/Pi cotransporter C-terminal-associated protein NaPi Cap1 NaPi-Cap1 NaPiCap1 NHERF 3 NHERF-3 NHERF3 NHRF3_HUMAN OTTHUMP00000015572 PDZ domain containing 1 PDZ domain containing protein 1 PDZ domain-containing protein 1 PDZD1 PDZK1 Sodium hydrogen exchanger regulatory factor 3 Sodium-hydrogen exchanger regulatory factor 3 1700023D20Rik 2610507N21Rik 4921513F16Rik AI267131 AI314638 AL022680 C terminal linking and modulating protein. |
|---|---|
| Molecular Weight(MW) | 57 KDa |
| Cellular Localization | Cytoplasm. Membrane. Associated with peripheral membranes. Localizes to the apical compartment of proximal tubular cells and to sinusoidal liver membranes. |
| SwissProt ID | Q5T2W1 |
|---|
Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- PDZK1 (ER1914-96) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD| Positive Control | Expression is limited to epithelial cells. Expressed in the kidney (brush border of proximal tubule), pancreas, liver, and small intestine. Expressed at a lower level in the adrenal cortex, testis and stomach. Overexpressed in breast, renal and lung carcinomas. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
