Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PEDF) Polyclonal Antibody, Unconjugated (ER1914-97) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PEDF) Polyclonal Antibody, Unconjugated (ER1914-97) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PEDF) Polyclonal Antibody, Unconjugated (ER1914-97) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig4: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-PEDF (ER1914-97) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD| Product Name | PEDF |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | pigment epithelium-derived factor Serpin-F1 Stromal cell-derived factor 3 SDF-3 Caspin Alpha 2 antiplasmin EPC 1 EPC1 PIG 35 PIG35 Pigment epithelium derived factor Proliferation inducing protein 35 SERPIN F1 Serpin peptidase inhibitor clade F member 1 SERPINF 1 SERPINF1 PEDF_HUMAN. |
| Product description | This gene encodes a member of the serpin family that does not display the serine protease inhibitory activity shown by many of the other serpin proteins. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI. |
| Immunogen | KLH conjugated synthetic peptide derived from human PEDF 101-200/417 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMousePigCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | pigment epithelium-derived factor Serpin-F1 Stromal cell-derived factor 3 SDF-3 Caspin Alpha 2 antiplasmin EPC 1 EPC1 PIG 35 PIG35 Pigment epithelium derived factor Proliferation inducing protein 35 SERPIN F1 Serpin peptidase inhibitor clade F member 1 SERPINF 1 SERPINF1 PEDF_HUMAN. |
|---|---|
| Molecular Weight(MW) | 46 KDa |
| Cellular Localization | Secreted. Melanosome. Enriched in stage I melanosomes. |
| SwissProt ID | P36955 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PEDF) Polyclonal Antibody, Unconjugated (ER1914-97) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PEDF) Polyclonal Antibody, Unconjugated (ER1914-97) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PEDF) Polyclonal Antibody, Unconjugated (ER1914-97) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig4: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-PEDF (ER1914-97) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD| Positive Control | Retinal pigment epithelial cells and blood plasma. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
