Application
Fig1: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-PER1 protein (ER1915-01) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 136 kD Observed band size: 136 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PER1 protein) Polyclonal Antibody, Unconjugated (ER1915-01) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | PER1 protein |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Circadian clock protein PERIOD 1 Circadian clock protein PERIOD1 Circadian pacemaker protein Rigui hPER 1 hPER hPER1 KIAA0482 MGC88021 PER 1 PER PER1 PER1 protein PER1_HUMAN Period 1 Period circadian protein homolog 1 Period drosophila homolog of Period homolog 1 Period1 RIGUI. |
| Product description | Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. Can bind heme (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human PER1 protein 751-850/1290 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Circadian clock protein PERIOD 1 Circadian clock protein PERIOD1 Circadian pacemaker protein Rigui hPER 1 hPER hPER1 KIAA0482 MGC88021 PER 1 PER PER1 PER1 protein PER1_HUMAN Period 1 Period circadian protein homolog 1 Period drosophila homolog of Period homolog 1 Period1 RIGUI. |
|---|---|
| Molecular Weight(MW) | 136 KDa |
| Cellular Localization | Nucleus (By similarity). Cytoplasm (By similarity). Note=Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2 (By similarity). |
| SwissProt ID | O15534 |
|---|
Application
Fig1: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-PER1 protein (ER1915-01) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 136 kD Observed band size: 136 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PER1 protein) Polyclonal Antibody, Unconjugated (ER1915-01) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, thymus, prostate, testis, ovary and small intestine. Highest level in skeletal muscle. Low level in kidney. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
