Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-PET112L (ER1915-02) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 58 kD Observed band size: 58 kDApplication
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-PET112L (ER1915-02) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 58 kD Observed band size: 58 kD| Product Name | PET112L |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cytochrome oxidase assembly factor PET112 homolog GATB_HUMAN GLU-ADT subunit B HSPC199 mitochondrial PET112 PET112 homolog (yeast) PET112-like Probable glutamyl-tRNA(Gln) amidotransferase subunit B. |
| Product description | Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). |
| Immunogen | KLH conjugated synthetic peptide derived from human PET112L 101-200/557 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogCow |
| Concentration | 1mg/ml |
| Alternative Names | Cytochrome oxidase assembly factor PET112 homolog GATB_HUMAN GLU-ADT subunit B HSPC199 mitochondrial PET112 PET112 homolog (yeast) PET112-like Probable glutamyl-tRNA(Gln) amidotransferase subunit B. |
|---|---|
| Molecular Weight(MW) | 58 KDa |
| Cellular Localization | Mitochondrion. |
| SwissProt ID | O75879 |
|---|
Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-PET112L (ER1915-02) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 58 kD Observed band size: 58 kD
Application
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-PET112L (ER1915-02) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 58 kD Observed band size: 58 kD| Positive Control | Predominantly expressed in tissues characterized by high rates of oxidative phosphorylation (OxPhos), including muscle and heart. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
