Application
Fig1: Sample: PC-3 Cell Lysate at 40 ug HepG2 Cell Lysate at 40 ug Primary: Anti- PFKFB2/PFK2 (ER1915-04) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 62 kDApplication
at 37℃ for 20 min; Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated(ER1915-04) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated(ER1915-04) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PFKFB2) Polyclonal Antibody, Unconjugated (ER1915-04) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | PFKFB2/PFK2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2 6-bisphosphatase 6-P2ase 2 6-P2ASE heart-type isozyme 6PF 2 K/Fru 2 6 P2ASE heart type isozyme 6PF-2-K/Fru-2 F262_HUMAN Fructose 2 6 bisphosphatase cardiac isozyme Fructose-2 PFK 2/FBPase 2 PFK/FBPase 2 PFKFB cardiac PFKFB2. |
| Product description | Synthesis and degradation of fructose 2,6-bisphosphate. |
| Immunogen | KLH conjugated synthetic peptide derived from human PFKFB2 51-150/505 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDog |
| Concentration | 1mg/ml |
| Alternative Names | 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2 6-bisphosphatase 6-P2ase 2 6-P2ASE heart-type isozyme 6PF 2 K/Fru 2 6 P2ASE heart type isozyme 6PF-2-K/Fru-2 F262_HUMAN Fructose 2 6 bisphosphatase cardiac isozyme Fructose-2 PFK 2/FBPase 2 PFK/FBPase 2 PFKFB cardiac PFKFB2. |
|---|---|
| Molecular Weight(MW) | 58 KDa |
| SwissProt ID | O60825 |
|---|
Application
Fig1: Sample: PC-3 Cell Lysate at 40 ug HepG2 Cell Lysate at 40 ug Primary: Anti- PFKFB2/PFK2 (ER1915-04) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 62 kD
Application
at 37℃ for 20 min; Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated(ER1915-04) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated(ER1915-04) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PFKFB2) Polyclonal Antibody, Unconjugated (ER1915-04) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Heart. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
