Application
Fig1: Sample: U87MG(Human) Cell Lysate at 30 ug Primary: Anti- Phospho1 (ER1915-07) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 35 kDApplication
Fig2: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-Phospho1 (ER1915-07) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 30 kD Observed band size: 30 kD| Product Name | Phospho1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EC 3.1.3.75 PHOP1_HUMAN Phosphatase orphan 1 Phosphatase, orphan 1 Phospho 1 Phosphoethanolamine/phosphocholine phosphatase. |
| Product description | Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization. |
| Immunogen | KLH conjugated synthetic peptide derived from human Phospho1 201-267/267 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | EC 3.1.3.75
PHOP1_HUMAN
Phosphatase orphan 1
Phosphatase orphan 1 Phospho 1 Phosphoethanolamine/phosphocholine phosphatase. |
|---|---|
| Molecular Weight(MW) | 30 KDa |
| SwissProt ID | Q8TCT1 |
|---|
Application
Fig1: Sample: U87MG(Human) Cell Lysate at 30 ug Primary: Anti- Phospho1 (ER1915-07) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 35 kD
Application
Fig2: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-Phospho1 (ER1915-07) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 30 kD Observed band size: 30 kD| Positive Control | Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
