Application
Fig1: Blank control(blue): Hela Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospholipase C beta 2/AF647 Conjugated antibody (ER1915-08-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.Application
Fig2: Sample: U-2OS(Human) Cell Lysate at 30 ug Primary: Anti- Phospholipase C beta 1 (ER1915-08) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 134 kD Observed band size: 134 kD| Product Name | Phospholipase C beta 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1-phosphatidylinositol-45-bisphosphate phosphodiesterase beta 1 1vphosphatidylinositol 4,5 bisphosphate phosphodiesterase beta 1 Phosphb Phosphoinositide phospholipase C Phosphoinositide phospholipase C-beta 1 Phospholipase C beta 1 (phosphoinositide-specific) Phospholipase C I PI PLC PLC 1 PLCβ1 PLC-154 PLC-beta-1 PLC-I PLC154 Plcb PLCB1 Plcb1 protein PLCbeta1. |
| Product description | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. |
| Immunogen | KLH conjugated synthetic peptide derived from human Phospholipase C beta 1 441-5401216 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | 1-phosphatidylinositol-45-bisphosphate phosphodiesterase beta 1
1vphosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 1 Phosphb Phosphoinositide phospholipase C Phosphoinositide phospholipase C-beta 1 Phospholipase C beta 1 (phosphoinositide-specific) Phospholipase C I PI PLC PLC 1 PLCβ1 PLC-154 PLC-beta-1 PLC-I PLC154 Plcb PLCB1 Plcb1 protein PLCbeta1. |
|---|---|
| Molecular Weight(MW) | 134 KDa |
| Cellular Localization | Cytoplasmic and Nuclear. |
| SwissProt ID | Q9NQ66 |
|---|
Application
Fig1: Blank control(blue): Hela Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospholipase C beta 2/AF647 Conjugated antibody (ER1915-08-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Application
Fig2: Sample: U-2OS(Human) Cell Lysate at 30 ug Primary: Anti- Phospholipase C beta 1 (ER1915-08) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 134 kD Observed band size: 134 kD| Application Notes | WB:1:500-2000: FC:1μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
