Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated (ER1915-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated (ER1915-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated (ER1915-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti-Phospholipase C beta 2 (ER1915-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kDApplication
Fig5: Blank control: Hela Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospholipase C beta 2/AF647 Conjugated antibody (ER1915-09-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.Application
Fig6: Sample: HL-60(Human) Cell Lysate at 30 ug Primary: Anti- Phospholipase C beta 2 (ER1915-09) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD| Product Name | Phospholipase C beta 2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLCβ2 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase beta-2 FLJ38135 Phosphoinositide phospholipase C-beta-2 Phospholipase C-beta-2 PLC-beta-2 Plcb2 PLCB2_HUMAN. |
| Product description | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. |
| Immunogen | KLH conjugated synthetic peptide derived from human Phospholipase C beta 2 401-500/1185 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IFFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: IF:1:50-200: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | PLCβ2
1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase beta-2 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase beta-2 FLJ38135 Phosphoinositide phospholipase C-beta-2 Phospholipase C-beta-2 PLC-beta-2 Plcb2 PLCB2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 130 KDa |
| SwissProt ID | Q00722 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated (ER1915-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated (ER1915-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated (ER1915-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti-Phospholipase C beta 2 (ER1915-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD
Application
Fig5: Blank control: Hela Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospholipase C beta 2/AF647 Conjugated antibody (ER1915-09-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Application
Fig6: Sample: HL-60(Human) Cell Lysate at 30 ug Primary: Anti- Phospholipase C beta 2 (ER1915-09) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: IF:1:50-200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
