Application
Fig1: Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PIBF Polyclonal Antibody, Unconjugated(ER1915-10) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Testis (Rat) Lysate at 40 ug Primary: Anti-PIBF (ER1915-10) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 83 kD Observed band size: 83 kD| Product Name | PIBF |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | progesterone induced blocking factor KIAA1008 PIBF 1 PIBF PIBF1 Progesterone immunomodulatory binding factor 1 Progesterone induced blocking factor 1 PIBF1_HUMAN RP11 505F3.1 CEP90 C13orf24 RP11-505F3.1. |
| Product description | Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect. |
| Immunogen | KLH conjugated synthetic peptide derived from human PIBF 551-650/756 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | progesterone induced blocking factor KIAA1008 PIBF 1 PIBF PIBF1 Progesterone immunomodulatory binding factor 1 Progesterone induced blocking factor 1 PIBF1_HUMAN RP11 505F3.1 CEP90 C13orf24 RP11-505F3.1. |
|---|---|
| Molecular Weight(MW) | 83 KDa |
| SwissProt ID | Q8WXW3 |
|---|
Application
Fig1: Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PIBF Polyclonal Antibody, Unconjugated(ER1915-10) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Testis (Rat) Lysate at 40 ug Primary: Anti-PIBF (ER1915-10) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 83 kD Observed band size: 83 kD| Positive Control | Expressed at highest levels in testis. Moderate expression is detected in spleen, thymus, prostate, ovary, small intestine, and colon. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
