Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PKC iota) Polyclonal Antibody, Unconjugated (ER1915-11) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-PKC iota (ER1915-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD| Product Name | PKC iota |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | aPKC lambda/iota aPKC-lambda/iota Atypical protein kinase C lambda/iota Atypical protein kinase C-lambda/iota DXS1179E KPCI_HUMAN MGC26534 nPKC iota nPKC-iota nPKCiota PKC lambda PKCI PKCiota PKClambda PRKC iota PRKC lambda/iota PRKC-lambda/iota PRKCI Protein kinase C iota Protein kinase C iota type. |
| Product description | Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. May play a role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. Functions in both pro- and anti-apoptotic pathways. Functions in the RAC1/ERK signaling required for transformed growth. Plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). |
| Immunogen | KLH conjugated synthetic peptide derived from human PKC iota/PKCI 181-280/596 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | aPKC lambda/iota aPKC-lambda/iota Atypical protein kinase C lambda/iota Atypical protein kinase C-lambda/iota DXS1179E KPCI_HUMAN MGC26534 nPKC iota nPKC-iota nPKCiota PKC lambda PKCI PKCiota PKClambda PRKC iota PRKC lambda/iota PRKC-lambda/iota PRKCI Protein kinase C iota Protein kinase C iota type. |
|---|---|
| Molecular Weight(MW) | 68 KDa |
| Cellular Localization | Cytoplasm. Membrane. Endosome. Nucleus. Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by cSrc, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Vesicular tubular clusters. Transported to VTCs through interaction with RAB2A. |
| SwissProt ID | P41743 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PKC iota) Polyclonal Antibody, Unconjugated (ER1915-11) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-PKC iota (ER1915-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD| Positive Control | Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
