Application
Fig1: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti- Podoplanin (ER1915-23) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kD Observed band size: 37 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Podoplanin) Polyclonal Antibody, Unconjugated (ER1915-23) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | Podoplanin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Podoplanin /gp36 Podoplanin Protein AGGRUS GLYCOPROTEIN 36 KD Podoplanin Glycoprotein 36 gp 36 GP 38 GP 40 gp36 GP38 GP40 HT1A 1 HT1A1 hT1alpha1 hT1alpha2 Lung type I cell membrane associated glycoprotein Lung type I cell membrane associated glycoprotein isoform a Lung type I cell membrane associated glycoprotein T1A 2 OTS 8 OTS8 OTTHUMP00000009640 OTTHUMP00000044504 PA2.26 PA2.26 antigen PDPN Podoplanin T1 alpha T1 ALPHA GENE T1A TIA 2 TIA2. |
| Product description | May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse Podoplanin 91-166/166 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Podoplanin /gp36
Podoplanin Protein
AGGRUS GLYCOPROTEIN 36 KD Podoplanin Glycoprotein 36 gp 36 GP 38 GP 40 gp36 GP38 GP40 HT1A 1 HT1A1 hT1alpha1 hT1alpha2 Lung type I cell membrane associated glycoprotein Lung type I cell membrane associated glycoprotein isoform a Lung type I cell membrane associated glycoprotein T1A 2 OTS 8 OTS8 OTTHUMP00000009640 OTTHUMP00000044504 PA2.26 PA2.26 antigen PDPN Podoplanin T1 alpha T1 ALPHA GENE T1A TIA 2 TIA2. |
|---|---|
| Molecular Weight(MW) | 15 KDa |
| Cellular Localization | Membrane; Plasma membrane. |
| SwissProt ID | Q62011 |
|---|
Application
Fig1: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti- Podoplanin (ER1915-23) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kD Observed band size: 37 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Podoplanin) Polyclonal Antibody, Unconjugated (ER1915-23) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Positive Control | Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
