Application
Fig1: Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-Pokemon/ZBTB7A (ER1915-25) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kDApplication
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Pokemon/ZBTB7A (ER1915-25) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kDApplication
Fig3: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-Pokemon/ZBTB7A (ER1915-25) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD| Product Name | Pokemon/ZBTB7A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Factor binding IST protein 1 Factor that binds to inducer of short transcripts protein 1 FBI-1 FBI1 HIV-1 1st-binding protein 1 Leukemia/lymphoma related factor LRF Pokemon TIP21 TTF-I interacting peptide 21 ZBTB7 ZBTB7A Zinc finger and BTB domain-containing protein 7A ZBT7A_HUMAN. |
| Product description | Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'. |
| Immunogen | KLH conjugated synthetic peptide derived from human Pokemon 151-250/569 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Factor binding IST protein 1
Factor that binds to inducer of short transcripts protein 1
FBI-1 FBI1 HIV-1 1st-binding protein 1 Leukemia/lymphoma related factor LRF Pokemon TIP21 TTF-I interacting peptide 21 ZBTB7 ZBTB7A Zinc finger and BTB domain-containing protein 7A ZBT7A_HUMAN. |
|---|---|
| Molecular Weight(MW) | 63 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | O95365 |
|---|
Application
Fig1: Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-Pokemon/ZBTB7A (ER1915-25) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD
Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Pokemon/ZBTB7A (ER1915-25) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD
Application
Fig3: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-Pokemon/ZBTB7A (ER1915-25) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD| Positive Control | Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
