Application
Fig1: Sample: Muscle (Rat) Lysate at 40 ug Muscle (Mouse) Lysate at 40 ug Primary: Anti-PPM1B/PP2CB (ER1915-29) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kDApplication
Fig2: Sample:Heart(Rat) Lysate at 40 ug Primary: Anti-PPM1B/PP2CB (ER1915-29) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD| Product Name | PPM1B/PP2CB |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PP2C beta PP2C beta X PP2C-beta PP2CB PP2Cbeta PPC2BETAX PPM 1B Ppm1b PPM1B_HUMAN Protein phosphatase 1B (formerly 2C) magnesium dependent beta isoform Protein phosphatase 1B Protein phosphatase 1B magnesium dependent beta isoform Protein phosphatase 2C beta isoform Protein phosphatase 2C isoform beta Protein phosphatase 2C like protein Protein phosphatase, Mg2+/Mn2+ dependent, 1B MGC21657. |
| Product description | Enzyme with a broad specificity. Dephosphorylates CDK2 and CDK6 in vitro. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences. |
| Immunogen | KLH conjugated synthetic peptide derived from human PPM1B 1-80/479 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | PP2C beta
PP2C beta X
PP2C-beta
PP2CB
PP2Cbeta
PPC2BETAX
PPM 1B
Ppm1b
PPM1B_HUMAN
Protein phosphatase 1B (formerly 2C) magnesium dependent beta isoform
Protein phosphatase 1B
Protein phosphatase 1B magnesium dependent beta isoform
Protein phosphatase 2C beta isoform
Protein phosphatase 2C isoform beta
Protein phosphatase 2C like protein
Protein phosphatase Mg2+/Mn2+ dependent 1B MGC21657. |
|---|---|
| Molecular Weight(MW) | 53 KDa |
| Cellular Localization | Cytosol, Membrane. |
| SwissProt ID | O75688 |
|---|
Application
Fig1: Sample: Muscle (Rat) Lysate at 40 ug Muscle (Mouse) Lysate at 40 ug Primary: Anti-PPM1B/PP2CB (ER1915-29) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD
Application
Fig2: Sample:Heart(Rat) Lysate at 40 ug Primary: Anti-PPM1B/PP2CB (ER1915-29) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD| Positive Control | Highly expressed in heart and skeletal muscle. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
