Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-PPM1D (ER1915-30) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kD| Product Name | PPM1D |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WPP-DOMAIN INTERACTING PROTEIN 1 EC 3.1.3.16 p53 induced protein phosphatase 1 PP2C delta PP2CD protein phosphatase 1D magnesium-dependent delta isoform Protein phosphatase 2C delta isoform Protein phosphatase 2C isoform delta Protein phosphatase magnesium dependent 1 delta WIP 1 WIP1 AV338790 PPM1D PPM1D_HUMAN. |
| Product description | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. |
| Immunogen | KLH conjugated synthetic peptide derived from human PPM1D 201-300/605 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCow |
| Concentration | 1mg/ml |
| Alternative Names | WPP-DOMAIN INTERACTING PROTEIN 1
EC 3.1.3.16
p53 induced protein phosphatase 1 PP2C delta PP2CD protein phosphatase 1D magnesium-dependent delta isoform Protein phosphatase 2C delta isoform Protein phosphatase 2C isoform delta Protein phosphatase magnesium dependent 1 delta WIP 1 WIP1 AV338790 PPM1D PPM1D_HUMAN. |
|---|---|
| Molecular Weight(MW) | 67 KDa |
| Cellular Localization | Cytosol, Nucleus. |
| SwissProt ID | O15297 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-PPM1D (ER1915-30) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kD| Positive Control | Hela(Human) Cell Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
