Application
Fig1: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-PPP1R10 (Bs- 11666R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 99 kD Observed band size: 99 kD| Product Name | PPP1R10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PNUTS CAT 53 CAT53 FB 19 FB19 FB19 protein MHC class I region proline rich protein CAT53 p99 Phosphatase 1 nuclear targeting subunit Phosphatase nuclear targeting subunit PNUTS PP1 binding protein of 114 kDa PP1R10 Protein phosphatase 1 regulatory (inhibitor) subunit 10 Protein phosphatase 1 regulatory subunit 10 Serine/threonine protein phosphatase 1 regulatory subunit 10 PP1RA_HUMAN. |
| Product description | PPP1R10 (serine/threonine protein phosphatase 1 regulatory subunit 10), also known as PNUTS, is a targeting subunit of protein phosphatase 1 (PP1). Furthermore, PPP1R10 acts as an inhibitor of PPP1CA and PPP1CC phosphatase activities. Its inhibitory activity on PPP1CA is only observed when PPP1R10 is phosphorylated by Protein Kinase A (PKA). PPP1R10 contains a zinc finger motif and is predominantly localised within the nucleus. Recently, PPP1R10 has been shown to target PP1 to GABA(C) receptors, shuttling from the nucleus to the cell membrane in cells co-expressing this receptor. This highlights a role for this protein in the regulation of synaptic signal transduction. PPP1R10 has also been shown to regulate the activity of p53 in response to hypoxia. |
| Immunogen | KLH conjugated synthetic peptide derived from human PPP1R10 151-250/940 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | PNUTS CAT 53 CAT53 FB 19 FB19 FB19 protein MHC class I region proline rich protein CAT53 p99 Phosphatase 1 nuclear targeting subunit Phosphatase nuclear targeting subunit PNUTS PP1 binding protein of 114 kDa PP1R10 Protein phosphatase 1 regulatory (inhibitor) subunit 10 Protein phosphatase 1 regulatory subunit 10 Serine/threonine protein phosphatase 1 regulatory subunit 10 PP1RA_HUMAN. |
|---|---|
| Molecular Weight(MW) | 99 KDa |
| Cellular Localization | Nucleus. Note=Found in discrete nucleoplasmic bodies and within nucleoli. Associates with chromatin during interphase, excluded from condensed chromosomes during early mitosis and is reloaded onto chromosomes at the late telophase |
| SwissProt ID | Q96QC0 |
|---|
Application
Fig1: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-PPP1R10 (Bs- 11666R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 99 kD Observed band size: 99 kD| Positive Control | Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
