Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-PPP2R1A (ER1915-32) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD| Product Name | PPP2R1A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PP2A PP2A subunit A PR65-alpha isoform PP2A subunit A R1 alpha isoform PP2A, subunit A, PR65-alpha isoform PP2A, subunit A, R1-alpha isoform PPP2R1A ppp2r1a-b PR65 PR65A Protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a Protein phosphatase 2 (formerly 2A), regulatory subunit A, Protein phosphatase 2 regulatory subunit A alpha isoform Protein phosphatase 2, 65-KD regulatory subunit A, alpha Protein phosphatase 2, regulatory subunit A, alpha isoform Protein phosphatase 2, structural/regulatory subunit A, alpha PPP2R1A Serine threonine protein phosphatase 65 kDa Serine/threonine protein phosphatase 2A 65 KDA regulatory subunit A alpha isoform Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Alpha isoform of regulatory subunit A, protein phosphatase DKFZp470L0914 Medium tumor antigen-associated 61 kDa protein 2AAA_HUMAN. |
| Product description | The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. |
| Immunogen | KLH conjugated synthetic peptide derived from human PPP2R1A 166-255/589 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | PP2A
PP2A subunit A PR65-alpha isoform
PP2A subunit A R1 alpha isoform
PP2A subunit A PR65-alpha isoform PP2A subunit A R1-alpha isoform PPP2R1A ppp2r1a-b PR65 PR65A Protein phosphatase 2 (formerly 2A) regulatory subunit A (PR 65) alpha isoform Protein phosphatase 2 (Formerly 2A) regulatory subunit A (PR 65) alpha isoform isoform CRA_a Protein phosphatase 2 (formerly 2A) regulatory subunit A Protein phosphatase 2 regulatory subunit A alpha isoform Protein phosphatase 2 65-KD regulatory subunit A alpha Protein phosphatase 2 regulatory subunit A alpha isoform Protein phosphatase 2 structural/regulatory subunit A alpha PPP2R1A Serine threonine protein phosphatase 65 kDa Serine/threonine protein phosphatase 2A 65 KDA regulatory subunit A alpha isoform Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Alpha isoform of regulatory subunit A protein phosphatase DKFZp470L0914 Medium tumor antigen-associated 61 kDa protein 2AAA_HUMAN. |
|---|---|
| Molecular Weight(MW) | 65 KDa |
| Cellular Localization | Cytoplasm. Chromosome, centromere. |
| SwissProt ID | P30153 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-PPP2R1A (ER1915-32) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
