Application
Fig1: Sample: A431 Cell (Human) Lysate at 40 ug Primary: Anti- PPP2R2B/PP2A-B55 (ER1915-33) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kDApplication
Fig2: Sample: HepG2 Cell (Human) Lysate at 30 ug Primary: Anti- PPP2R2B/PP2A-B55 (ER1915-33) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kDApplication
Fig3: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti- PPP2R2B/PP2A-B55 (ER1915-33) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kDApplication
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti- PPP2R2B (ER1915-33) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD| Product Name | PPP2R2B/PP2A-B55 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2ABB_HUMAN B55 beta Beta isoform of regulatory subunit B55 protein phosphatase 2 MGC24888 PP2A B Subunit B Alpha Isoform PP2A B55beta PP2A PR55B PP2A subunit B B beta isoform PP2A subunit B B55 beta isoform PP2A subunit B isoform B55 beta PP2A subunit B isoform B55-beta PP2A subunit B isoform beta PP2A subunit B isoform PR55 beta PP2A subunit B isoform PR55-beta PP2A subunit B isoform R2 beta PP2A subunit B isoform R2-beta PP2A subunit B PR55 beta isoform PP2A subunit B R2 beta isoform Ppp2r2b PR2AB beta PR2AB55 beta PR2APR55 beta PR52B PR55 beta Protein phosphatase 2 (formerly 2A) regulatory subunit B (PR 52) beta isoform Protein phosphatase 2 (formerly 2A) regulatory subunit B beta isoform Protein phosphatase 2 regulatory subunit B Protein phosphatase 2 regulatory subunit B beta isoform SCA 12 SCA12 Serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B beta isoform Serine/Threonine Protein Phosphatase 2A 56kDa Regulatory Subunit Alpha Isoform Serine/threonine protein phosphatase 2A neuronal isoform Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform. |
| Product description | The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity (By similarity). Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance. |
| Immunogen | KLH conjugated synthetic peptide derived from human PPP2R2B/PP2A-B55 51-130/443 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | 2ABB_HUMAN B55 beta Beta isoform of regulatory subunit B55 protein phosphatase 2 MGC24888 PP2A B Subunit B Alpha Isoform PP2A B55beta PP2A PR55B PP2A subunit B B beta isoform PP2A subunit B B55 beta isoform PP2A subunit B isoform B55 beta PP2A subunit B isoform B55-beta PP2A subunit B isoform beta PP2A subunit B isoform PR55 beta PP2A subunit B isoform PR55-beta PP2A subunit B isoform R2 beta PP2A subunit B isoform R2-beta PP2A subunit B PR55 beta isoform PP2A subunit B R2 beta isoform Ppp2r2b PR2AB beta PR2AB55 beta PR2APR55 beta PR52B PR55 beta Protein phosphatase 2 (formerly 2A) regulatory subunit B (PR 52) beta isoform Protein phosphatase 2 (formerly 2A) regulatory subunit B beta isoform Protein phosphatase 2 regulatory subunit B Protein phosphatase 2 regulatory subunit B beta isoform SCA 12 SCA12 Serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B beta isoform Serine/Threonine Protein Phosphatase 2A 56kDa Regulatory Subunit Alpha Isoform Serine/threonine protein phosphatase 2A neuronal isoform Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform. |
|---|---|
| Molecular Weight(MW) | 52 KDa |
| Cellular Localization | Cytoplasm. cytoskeleton. Membrane and Cytoplasm. Mitochondrion. Mitochondrion outer membrane. Under basal conditions, localizes to both cytosolic and mitochondrial compartments. Relocalizes from the cytosolic to the mitochondrial compartment during apoptosis. Its targeting to the outer mitochondrial membrane (OMM) involves an association with import receptors of the TOM complex and is required to promote proapoptotic activity. |
| SwissProt ID | Q00005 |
|---|
Application
Fig1: Sample: A431 Cell (Human) Lysate at 40 ug Primary: Anti- PPP2R2B/PP2A-B55 (ER1915-33) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kD
Application
Fig2: Sample: HepG2 Cell (Human) Lysate at 30 ug Primary: Anti- PPP2R2B/PP2A-B55 (ER1915-33) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kD
Application
Fig3: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti- PPP2R2B/PP2A-B55 (ER1915-33) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kD
Application
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti- PPP2R2B (ER1915-33) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD| Positive Control | Brain. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
