Application
Fig1: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- PPP2R5E (ER1915-37) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kDApplication
Fig2: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti- PPP2R5E (ER1915-37) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PPP2R5E) Polyclonal Antibody, Unconjugated (ER1915-37) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Epithelium (Mouse) Lysate at 40 ug Primary: Anti-PPP2R5E(ER1915-37)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55kD Observed band size: 55kD| Product Name | PPP2R5E |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2A5E_HUMAN Epsilon isoform of regulatory subunit B56 protein phosphatase 2A PP2A B subunit B' epsilon PP2A B subunit B' epsilon isoform PP2A B subunit B56 epsilon PP2A B subunit B56 epsilon isoform PP2A B subunit isoform B''-epsilon PP2A B subunit isoform B'-epsilon PP2A B subunit isoform B56-epsilon PP2A B subunit isoform PR61-epsilon PP2A B subunit isoform R5-epsilon PP2A B subunit PR61 epsilon PP2A B subunit PR61 epsilon isoform PP2A B subunit R5 epsilon PP2A B subunit R5 epsilon isoform PPP2R5E Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform Protein phosphatase 2 regulatory subunit B' epsilon isoform Regulatory subunit B of protein phosphatase 2 epsilon isoform Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon. |
| Product description | The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. |
| Immunogen | KLH conjugated synthetic peptide derived from human PPP2R5E 121-220/467 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatHorse |
| Concentration | 1mg/ml |
| Alternative Names | 2A5E_HUMAN Epsilon isoform of regulatory subunit B56 protein phosphatase 2A PP2A B subunit B' epsilon PP2A B subunit B' epsilon isoform PP2A B subunit B56 epsilon PP2A B subunit B56 epsilon isoform PP2A B subunit isoform B''-epsilon PP2A B subunit isoform B'-epsilon PP2A B subunit isoform B56-epsilon PP2A B subunit isoform PR61-epsilon PP2A B subunit isoform R5-epsilon PP2A B subunit PR61 epsilon PP2A B subunit PR61 epsilon isoform PP2A B subunit R5 epsilon PP2A B subunit R5 epsilon isoform PPP2R5E Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform Protein phosphatase 2 regulatory subunit B' epsilon isoform Regulatory subunit B of protein phosphatase 2 epsilon isoform Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon. |
|---|---|
| Molecular Weight(MW) | 55 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q16537 |
|---|
Application
Fig1: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- PPP2R5E (ER1915-37) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Application
Fig2: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti- PPP2R5E (ER1915-37) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PPP2R5E) Polyclonal Antibody, Unconjugated (ER1915-37) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Epithelium (Mouse) Lysate at 40 ug Primary: Anti-PPP2R5E(ER1915-37)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55kD Observed band size: 55kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
