Application
Fig1: Sample: A549 (Human)Cell Lysate at 40 ug Primary: Anti-PPP3CC(ER1915-38)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 58kD Observed band size: 58kDApplication
Fig2: Sample: Testis(Mouse)Lysate at 40 ug Primary: Anti-PPP3CC(ER1915-38)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 58kD Observed band size: 58kDApplication
Fig3: Sample: DU145(Human) Cell Lysate at 40 ug Primary: Anti-PPP3CC (ER1915-38) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kDApplication
Fig4: Sample: Hela(Human) Cell Lysate at 40 ug Primary: Anti-PPP3CC (ER1915-38) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kDApplication
Fig5: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PPP3CC) Polyclonal Antibody, Unconjugated (ER1915-38) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Product Name | PPP3CC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | calcineurin A gamma CALCINEURIN A3 Calcineurin Calcineurin testis specific catalytic subunit Calmodulin dependent calcineurin A subunit gamma isoform Calmodulin-dependent calcineurin A subunit gamma isoform CALNA3 CAM PRP catalytic subunit CAM-PRP catalytic subunit CNA3 pp2b gamma PP2BC_HUMAN PP2Bgamma PPP3CC protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform (calcineurin A gamma) protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform testis-specific catalytic subunit. |
| Product description | Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. |
| Immunogen | KLH conjugated synthetic peptide derived from human PPP3CC 51-150/512 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | calcineurin A gamma CALCINEURIN A3 Calcineurin Calcineurin testis specific catalytic subunit Calmodulin dependent calcineurin A subunit gamma isoform Calmodulin-dependent calcineurin A subunit gamma isoform CALNA3 CAM PRP catalytic subunit CAM-PRP catalytic subunit CNA3 pp2b gamma PP2BC_HUMAN PP2Bgamma PPP3CC protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform (calcineurin A gamma) protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform testis-specific catalytic subunit. |
|---|---|
| Molecular Weight(MW) | 58 KDa |
| SwissProt ID | P48454 |
|---|
Application
Fig1: Sample: A549 (Human)Cell Lysate at 40 ug Primary: Anti-PPP3CC(ER1915-38)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 58kD Observed band size: 58kD
Application
Fig2: Sample: Testis(Mouse)Lysate at 40 ug Primary: Anti-PPP3CC(ER1915-38)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 58kD Observed band size: 58kD
Application
Fig3: Sample: DU145(Human) Cell Lysate at 40 ug Primary: Anti-PPP3CC (ER1915-38) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD
Application
Fig4: Sample: Hela(Human) Cell Lysate at 40 ug Primary: Anti-PPP3CC (ER1915-38) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD
Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PPP3CC) Polyclonal Antibody, Unconjugated (ER1915-38) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Positive Control | Testis. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
