Application
Fig1: Blank control: Raji. Primary Antibody (green line): Rabbit Anti-presenilin 1 antibody (ER1915-40) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: K562 Cell Lysate at 40 ug Primary: Anti- presenilin1 (ER1915-40) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34/52 kD Observed band size: 48 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (presenilin 1) Polyclonal Antibody, Unconjugated (ER1915-40) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig4: Sample: K562 Cell (Human) Lysate at 40 ug HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-presenilin 1 (ER1915-40) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34/52 kD Observed band size: 50 kD| Product Name | presenilin 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Presenilin-1 NTF subunit AD 3 AD3 Ad3h Alzheimer Disease 3 EC 3.4.23. FAD Homo Sapiens Clone CC44 Senilin 1 Presenilin 1 Alzheimer disease 3 Presenilin 1 Presenilin1 Protein S182 PS 1 PS-1 PS1 PSEN 1 PSEN1 PSN 1 PSN1 PSN1_HUMAN PSNL 1 PSNL1 S182 S182 Protein Senilin 1 Senilin1. |
| Product description | Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis. |
| Immunogen | KLH conjugated synthetic peptide derived from human Presenilin-1 NTF subunit 10-80/467 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1ug/test: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Presenilin-1 NTF subunit AD 3 AD3 Ad3h Alzheimer Disease 3 EC 3.4.23. FAD Homo Sapiens Clone CC44 Senilin 1 Presenilin 1 Alzheimer disease 3 Presenilin 1 Presenilin1 Protein S182 PS 1 PS-1 PS1 PSEN 1 PSEN1 PSN 1 PSN1 PSN1_HUMAN PSNL 1 PSNL1 S182 S182 Protein Senilin 1 Senilin1. |
|---|---|
| Molecular Weight(MW) | 34/52 KDa |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cell surface. Note=Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils. |
| SwissProt ID | P49768 |
|---|
Application
Fig1: Blank control: Raji. Primary Antibody (green line): Rabbit Anti-presenilin 1 antibody (ER1915-40) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: K562 Cell Lysate at 40 ug Primary: Anti- presenilin1 (ER1915-40) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34/52 kD Observed band size: 48 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (presenilin 1) Polyclonal Antibody, Unconjugated (ER1915-40) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig4: Sample: K562 Cell (Human) Lysate at 40 ug HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-presenilin 1 (ER1915-40) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34/52 kD Observed band size: 50 kD| Positive Control | Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1ug/test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
