Application
Fig1: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-PRLR (ER1915-43) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 67 kD Observed band size: 67 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PRLR) Polyclonal Antibody, Unconjugated (ER1915-43) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PRLR Polyclonal Antibody, Unconjugated(ER1915-43) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | PRLR |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lactogen receptor MGC105486 OPR Pr-1 Pr-3 PRL R PRL-R PRLR Prlr-rs1 PRLR_HUMAN Prolactin receptor a Prolactin receptor Prolactin receptor delta 7/11 RATPRLR Secreted prolactin binding protein Truncated testis-specific box 1-C prolactin receptor wu:fj65c07 AI987712 CLONE SPM213 CPRLP Delta 4-delta 7/11 truncated prolactin receptor Delta 4-SF1b truncated prolactin receptor hPRL receptor hPRLrI. |
| Product description | This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling. |
| Immunogen | KLH conjugated synthetic peptide derived from human Prolactin Receptor 251-350/622 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Lactogen receptor MGC105486 OPR Pr-1 Pr-3 PRL R PRL-R PRLR Prlr-rs1 PRLR_HUMAN Prolactin receptor a Prolactin receptor Prolactin receptor delta 7/11 RATPRLR Secreted prolactin binding protein Truncated testis-specific box 1-C prolactin receptor wu:fj65c07 AI987712 CLONE SPM213 CPRLP Delta 4-delta 7/11 truncated prolactin receptor Delta 4-SF1b truncated prolactin receptor hPRL receptor hPRLrI. |
|---|---|
| Molecular Weight(MW) | 67 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Isoform 7: Secreted. |
| SwissProt ID | P16471 |
|---|
Application
Fig1: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-PRLR (ER1915-43) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 67 kD Observed band size: 67 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PRLR) Polyclonal Antibody, Unconjugated (ER1915-43) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PRLR Polyclonal Antibody, Unconjugated(ER1915-43) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Expressed in breast, placenta, kidney, liver and pancreas. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
