Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PRODH) Polyclonal Antibody, Unconjugated (ER1915-44) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- PRODH (ER1915-44) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD| Product Name | PRODH |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSPOX2 P53 induced gene 6 protein PIG6 PRODH 1 PRODH 2 PRODH1 PRODH2 Proline dehydrogenase proline dehydrogenase (oxidase) 1 proline dehydrogenase (proline oxidase) Proline oxidase, mitochondrial precursor SCZD4 TP53I6 tumor protein p53 inducible protein 6 PROD_HUMAN. |
| Product description | Converts proline to delta-1-pyrroline-5-carboxylate. |
| Immunogen | KLH conjugated synthetic peptide derived from human PRODH 141-240/600 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatHorse |
| Concentration | 1mg/ml |
| Alternative Names | HSPOX2
P53 induced gene 6 protein
PIG6
PRODH 1
PRODH 2
PRODH1
PRODH2
Proline dehydrogenase
proline dehydrogenase (oxidase) 1
proline dehydrogenase (proline oxidase)
Proline oxidase mitochondrial precursor SCZD4 TP53I6 tumor protein p53 inducible protein 6 PROD_HUMAN. |
|---|---|
| Molecular Weight(MW) | 68 KDa |
| Cellular Localization | Mitochondrion matrix. |
| SwissProt ID | O43272 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PRODH) Polyclonal Antibody, Unconjugated (ER1915-44) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- PRODH (ER1915-44) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD| Positive Control | Expressed in lung, skeletal muscle and brain, to a lesser extent in heart and kidney, and weakly in liver, placenta and pancreas. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
