Application
dded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HRG beta1 Polyclonal Antibody, Unconjugated(ER1915-45) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Raji Cell Lysate at 30ug; Primary: Anti-HRG beta1 (ER1915-45) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size :71 kD Observed band size :71 kDApplication
Fig3: ue/cell: Mouse ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- HRG Beta1 Polyclonal Antibody, Unconjugated(ER1915-45) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Pro-neuregulin-1, membrane-bound isoform) Polyclonal Antibody, Unconjugated (ER1915-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig5: Blank control:A549. Primary Antibody (green line): Rabbit Anti-Pro-neuregulin-1, membrane-bound isoform antibody (ER1915-45) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig6: Blank control:A549. Primary Antibody (green line): Rabbit Anti-Pro-neuregulin-1, membrane-bound isoform antibody (ER1915-45) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | Pro-neuregulin-1, membrane-bound isoform |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Acetylcholine receptor-inducing activity ARIA Breast cancer cell differentiation factor p45 CHICK, homolog of GGF GGF2 Glial growth factor Heregulin heregulin, alpha (45kD, ERBB2 p185-activator) heregulin, alpha HGL HRG HRG1 HRGA MST131 NDF Neu differentiation factor neuregulin 1 type IV beta 1a neuregulin 1 type IV beta 3 NRG 1 nrg1 NRG1 beta NRG1_HUMAN Pro-neuregulin-1, membrane-bound isoform Pro-NRG1 Sensory and motor neuron-derived factor SMDF NRG1_MOUSE. |
| Product description | Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse HRG Beta1 221-320/636 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:3ug/test: |
|
| Species Reactivity | HumanMouseRatChickenDogRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Acetylcholine receptor-inducing activity
ARIA
Breast cancer cell differentiation factor p45
CHICK homolog of GGF GGF2 Glial growth factor Heregulin heregulin alpha (45kD ERBB2 p185-activator) heregulin alpha HGL HRG HRG1 HRGA MST131 NDF Neu differentiation factor neuregulin 1 type IV beta 1a neuregulin 1 type IV beta 3 NRG 1 nrg1 NRG1 beta NRG1_HUMAN Pro-neuregulin-1 membrane-bound isoform Pro-NRG1 Sensory and motor neuron-derived factor SMDF NRG1_MOUSE. |
|---|---|
| Molecular Weight(MW) | 71 KDa |
| Cellular Localization | Pro-neuregulin-1, membrane-bound isoform: Cell membrane; Single-pass type I membrane protein. Note=Does not seem to be active. ;Neuregulin-1: Secreted. ;Isoform 8: Nucleus. Note=May be nuclear. ;Isoform 9: Secreted. Note=Has a signal peptide. ;Isoform 10: Membrane; Single-pass type I membrane protein. Note=May possess an internal uncleaved signal sequence. |
| SwissProt ID | Q02297 |
|---|
Application
dded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HRG beta1 Polyclonal Antibody, Unconjugated(ER1915-45) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Raji Cell Lysate at 30ug; Primary: Anti-HRG beta1 (ER1915-45) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size :71 kD Observed band size :71 kD
Application
Fig3: ue/cell: Mouse ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- HRG Beta1 Polyclonal Antibody, Unconjugated(ER1915-45) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Pro-neuregulin-1, membrane-bound isoform) Polyclonal Antibody, Unconjugated (ER1915-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig5: Blank control:A549. Primary Antibody (green line): Rabbit Anti-Pro-neuregulin-1, membrane-bound isoform antibody (ER1915-45) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig6: Blank control:A549. Primary Antibody (green line): Rabbit Anti-Pro-neuregulin-1, membrane-bound isoform antibody (ER1915-45) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:3ug/test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
