Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PGDH) Polyclonal Antibody, Unconjugated (ER1915-46) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: LOVO(Human) Cell Lysate at 30 ug Primary: Anti-Prostaglandin dehydrogenase 1 (ER1915-46) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kDApplication
Fig3: Sample: SW480(Human) Cell Lysate at 30 ug Primary: Anti-Prostaglandin dehydrogenase 1 (ER1915-46) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD| Product Name | Prostaglandin dehydrogenase 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HPGD Hydroxyprostaglandin dehydrogenase 15 (NAD) NAD+ dependent 15 hydroxyprostaglandin dehydrogenase PGDH PGDH_HUMAN PGDH1 Prostaglandin dehydrogenase 1 SDR36C1 Short chain dehydrogenase/reductase family 36C,member 1 15 hydroxyprostaglandin dehydrogenase [NAD+] 15 PGDH 15-hydroxyprostaglandin dehydrogenase [NAD+] 15-PGDH 15PGDH. |
| Product description | Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells. |
| Immunogen | KLH conjugated synthetic peptide derived from human Prostaglandin dehydrogenase 1 55-160/266 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | HPGD
Hydroxyprostaglandin dehydrogenase 15 (NAD)
NAD+ dependent 15 hydroxyprostaglandin dehydrogenase
PGDH
PGDH_HUMAN
PGDH1
Prostaglandin dehydrogenase 1
SDR36C1
Short chain dehydrogenase/reductase family 36C member 1 15 hydroxyprostaglandin dehydrogenase [NAD+] 15 PGDH 15-hydroxyprostaglandin dehydrogenase [NAD+] 15-PGDH 15PGDH. |
|---|---|
| Molecular Weight(MW) | 29 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P15428 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PGDH) Polyclonal Antibody, Unconjugated (ER1915-46) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: LOVO(Human) Cell Lysate at 30 ug Primary: Anti-Prostaglandin dehydrogenase 1 (ER1915-46) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD
Application
Fig3: Sample: SW480(Human) Cell Lysate at 30 ug Primary: Anti-Prostaglandin dehydrogenase 1 (ER1915-46) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD| Positive Control | Detected in colon epithelium (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
