Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Prostaglandin E Receptor EP1 (ER1915-47) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 45 kDApplication
Fig2: Sample: Brain (Rat) Lysate at 40 ug Primary: Anti-Prostaglandin E Receptor EP1 (ER1915-47) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 42 kD Observed band size: 42 kDApplication
Fig3: Blank control (Black line): Mouse blood (Black). Primary Antibody (green line): Rabbit Anti-Prostaglandin E Receptor EP1 antibody (ER1915-47) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | Prostaglandin E Receptor EP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Prostaglandin E Receptor EP1 EP1 PGE receptor EP1 subtype PGE2 receptor EP1 subtype Prostaglandin E receptor 1 subtype EP1 42kDa Prostaglandin E receptor 1 subtype EP1 Prostaglandin E2 receptor EP1 subtype Prostanoid EP1 receptor PE2R1_HUMAN. |
| Immunogen | KLH conjugated synthetic peptide derived from human PTGER1/Prostaglandin E Receptor EP1 61-160/402 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatDog |
| Concentration | 1mg/ml |
| Alternative Names | Prostaglandin E Receptor EP1 EP1 PGE receptor EP1 subtype PGE2 receptor EP1 subtype Prostaglandin E receptor 1 subtype EP1 42kDa Prostaglandin E receptor 1 subtype EP1 Prostaglandin E2 receptor EP1 subtype Prostanoid EP1 receptor PE2R1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 42 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P34995 |
|---|
Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Prostaglandin E Receptor EP1 (ER1915-47) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 45 kD
Application
Fig2: Sample: Brain (Rat) Lysate at 40 ug Primary: Anti-Prostaglandin E Receptor EP1 (ER1915-47) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 42 kD Observed band size: 42 kD
Application
Fig3: Blank control (Black line): Mouse blood (Black). Primary Antibody (green line): Rabbit Anti-Prostaglandin E Receptor EP1 antibody (ER1915-47) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Abundant in kidney. Lower level expression in lung, skeletal muscle and spleen, lowest expression in testis and not detected in liver brain and heart. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
