Application
Fig1: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-Protective protein (ER1915-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kDApplication
Fig2: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti-Protective protein (ER1915-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Protective protein,Cathepsin A) Polyclonal Antibody, Unconjugated (ER1915-50) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | Protective protein/Cathepsin A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cathepsin A CTSA BETA GALACTOSIDASE PROTECTIVE PROTEIN Carboxypeptidase C Glactosialidosis GLB2 Goldberg Syndrome GSL Lysosomal protective protein NEURAMINIDASE BETA GALACTOSIDASE EXPRESSION NGBE NGBE PPCA PPGB Protective protein for beta galactosidase PPGB_HUMAN. |
| Product description | Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins. |
| Immunogen | KLH conjugated synthetic peptide derived from human Protective protein 401-480/480 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | Cathepsin A CTSA BETA GALACTOSIDASE PROTECTIVE PROTEIN Carboxypeptidase C Glactosialidosis GLB2 Goldberg Syndrome GSL Lysosomal protective protein NEURAMINIDASE BETA GALACTOSIDASE EXPRESSION NGBE NGBE PPCA PPGB Protective protein for beta galactosidase PPGB_HUMAN. |
|---|---|
| Molecular Weight(MW) | 51 KDa |
| Cellular Localization | Lysosome. |
| SwissProt ID | P10619 |
|---|
Application
Fig1: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-Protective protein (ER1915-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
Application
Fig2: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti-Protective protein (ER1915-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Protective protein,Cathepsin A) Polyclonal Antibody, Unconjugated (ER1915-50) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
