Application
Fig1: Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PRSS10 Polyclonal Antibody, Unconjugated(ER1915-52) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | PRSS10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Epitheliasin PRSS10 Serine protease 10 TMPRSS2 TMPRSS2 ERG FUSION GENE, INCLUDED TMPRSS2 ETV1 FUSION GENE, INCLUDED TMPS2_HUMAN Transmembrane protease serine 2 catalytic chain Transmembrane protease, serine 2 Transmembrane protease, serine 2, EC 3.4.219 Transmembrane protease, serine 2, EC 3.4.219 D16Ertd61e. |
| Product description | Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland. |
| Immunogen | KLH conjugated synthetic peptide derived from human PRSS10 301-400/492 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | Epitheliasin
PRSS10
Serine protease 10
TMPRSS2
TMPRSS2 ERG FUSION GENE INCLUDED TMPRSS2 ETV1 FUSION GENE INCLUDED TMPS2_HUMAN Transmembrane protease serine 2 catalytic chain Transmembrane protease serine 2 Transmembrane protease serine 2 EC 3.4.219 Transmembrane protease serine 2 EC 3.4.219 D16Ertd61e. |
|---|---|
| Molecular Weight(MW) | 28 KDa |
| Cellular Localization | Cell membrane and Secreted. Activated by cleavage and secreted. |
| SwissProt ID | O15393 |
|---|
Application
Fig1: Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PRSS10 Polyclonal Antibody, Unconjugated(ER1915-52) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
