Application
Fig1: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-Prostaglandin E Receptor EP2 (ER1915-56) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kDApplication
Fig2: Blank control(blue): A549(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Prostaglandin E Receptor EP2/AF647 Conjugated antibody (ER1915-56-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.| Product Name | PTGER2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EP 2 EP2 Prostaglandin E Receptor EP2 Human prostaglandin E2 receptor PE2R2_HUMAN PGE receptor EP2 subtype PGE2 receptor EP2 subtype Prostaglandin E receptor 2 EP2 subtype Prostaglandin E receptor 2 subtype EP2 53kDa Prostaglandin E receptor 2 subtype EP2 Prostaglandin E2 receptor Prostaglandin E2 receptor EP2 subtype Prostanoid EP2 receptor PTGER 2 PTGER2. |
| Product description | Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle. |
| Immunogen | KLH conjugated synthetic peptide derived from human Prostaglandin E Receptor EP2 271-358/358 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | EP 2 EP2 Prostaglandin E Receptor EP2 Human prostaglandin E2 receptor PE2R2_HUMAN PGE receptor EP2 subtype PGE2 receptor EP2 subtype Prostaglandin E receptor 2 EP2 subtype Prostaglandin E receptor 2 subtype EP2 53kDa Prostaglandin E receptor 2 subtype EP2 Prostaglandin E2 receptor Prostaglandin E2 receptor EP2 subtype Prostanoid EP2 receptor PTGER 2 PTGER2. |
|---|---|
| Molecular Weight(MW) | 40 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P43116 |
|---|
Application
Fig1: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-Prostaglandin E Receptor EP2 (ER1915-56) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kD
Application
Fig2: Blank control(blue): A549(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Prostaglandin E Receptor EP2/AF647 Conjugated antibody (ER1915-56-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.| Positive Control | Placenta and lung. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
