Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PVRL1) Polyclonal Antibody, Unconjugated (ER1915-63) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-PVRL1 (ER1915-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 57 kDApplication
Fig3: Sample: Testis(Mouse) Lysate at 40 ug Primary: Anti-PVRL1 (ER1915-63) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kDApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PVRL1) Polyclonal Antibody, Unconjugated (ER1915-63) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | PVRL1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD111 CD111 antigen CLPED1 ectodermal dysplasia 4 (Margarita Island type) ED4 Herpes virus entry mediator C Herpesvirus entry mediator C Herpesvirus Ig like receptor Herpesvirus Ig-like receptor HIgR HveC Nectin 1 Nectin-1 Nectin1 OFC7 OROFACIAL CLEFT 7 Poliovirus receptor related protein 1 poliovirus receptor-like 1 Poliovirus receptor-related protein 1 PRR PRR1 PVRL 1 PVRL1 PVRL1_HUMAN PVRR PVRR1 SK-12. |
| Product description | Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4. |
| Immunogen | KLH conjugated synthetic peptide derived from human PVRL1/CD111/Nectin1 31-130/517 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | CD111 CD111 antigen CLPED1 ectodermal dysplasia 4 (Margarita Island type) ED4 Herpes virus entry mediator C Herpesvirus entry mediator C Herpesvirus Ig like receptor Herpesvirus Ig-like receptor HIgR HveC Nectin 1 Nectin-1 Nectin1 OFC7 OROFACIAL CLEFT 7 Poliovirus receptor related protein 1 poliovirus receptor-like 1 Poliovirus receptor-related protein 1 PRR PRR1 PVRL 1 PVRL1 PVRL1_HUMAN PVRR PVRR1 SK-12. |
|---|---|
| Molecular Weight(MW) | 54 KDa |
| Cellular Localization | Isoform Alpha: Cell membrane; Single-pass type I membrane protein. Isoform Delta: Cell membrane; Single-pass type I membrane protein. Isoform Gamma: Secreted. |
| SwissProt ID | Q15223 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PVRL1) Polyclonal Antibody, Unconjugated (ER1915-63) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-PVRL1 (ER1915-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 57 kD
Application
Fig3: Sample: Testis(Mouse) Lysate at 40 ug Primary: Anti-PVRL1 (ER1915-63) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PVRL1) Polyclonal Antibody, Unconjugated (ER1915-63) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
