Application
Fig1: Sample: U2OS(Human) Cell Lysate at 30 ug Primary: Anti- QK1 (ER1915-66) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 40 kD| Product Name | QK1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HKQ Homolog of mouse quaking QKI KH domain RNA binding protein Hqk HQK1 HqkI Protein quaking QK QK1 QK3 QKI QKI_HUMAN QKI1 Quaking homolog Quaking homolog KH domain RNA binding Quaking homolog KH domain RNA binding mouse Quaking isoform 1 Quaking protein RNA binding protein HQK. |
| Product description | RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor. |
| Immunogen | KLH conjugated synthetic peptide derived from human QK1 220/341/341 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | HKQ Homolog of mouse quaking QKI KH domain RNA binding protein Hqk HQK1 HqkI Protein quaking QK QK1 QK3 QKI QKI_HUMAN QKI1 Quaking homolog Quaking homolog KH domain RNA binding Quaking homolog KH domain RNA binding mouse Quaking isoform 1 Quaking protein RNA binding protein HQK. |
|---|---|
| Molecular Weight(MW) | 38 KDa |
| Cellular Localization | Nucleus. Cytoplasm. |
| SwissProt ID | Q96PU8 |
|---|
Application
Fig1: Sample: U2OS(Human) Cell Lysate at 30 ug Primary: Anti- QK1 (ER1915-66) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 40 kD| Positive Control | Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
