Application
Fig1: Sample: 293T Cell (Human) Lysate at 40 ug Hela Cell (Human) Lysate at 40 ug Primary: Anti- RAB11-FIP2 (ER1915-67) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kDApplication
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- RAB11-FIP2 (ER1915-67) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD| Product Name | RAB11-FIP2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAB11FIP2 KIAA0941 NRip11 RAB11 family interacting protein 2 (class I) Rab11 family-interacting protein 2 RAB11 FIP2 long isoform Rab11-FIP2 RFIP2 RFIP2_HUMAN. |
| Product description | A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity. |
| Immunogen | KLH conjugated synthetic peptide derived from human RAB11-FIP2 331-430/512 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitGuinea PigCat |
| Concentration | 1mg/ml |
| Alternative Names | RAB11FIP2
KIAA0941
NRip11 RAB11 family interacting protein 2 (class I) Rab11 family-interacting protein 2 RAB11 FIP2 long isoform Rab11-FIP2 RFIP2 RFIP2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 58 KDa |
| Cellular Localization | Cell membrane. Recycling endosome membrane. Translocates with RAB11A from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane. |
| SwissProt ID | Q7L804 |
|---|
Application
Fig1: Sample: 293T Cell (Human) Lysate at 40 ug Hela Cell (Human) Lysate at 40 ug Primary: Anti- RAB11-FIP2 (ER1915-67) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD
Application
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- RAB11-FIP2 (ER1915-67) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD| Positive Control | 293T Cell (Human) Lysate, Hela Cell (Human) Lysate, Cerebrum (Mouse) Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
