Application
Fig1: Sample: U251 Cell Lysate at 30 ug Primary: Anti- RANK (ER1915-70) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63-70 kD Observed band size: 63 kDApplication
Fig2: Blank control(blue):Jurkat cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-RANK antibody(ER1915-70), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Application
Fig3: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-RANK (ER1915-70) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63-70 kD Observed band size: 70 kD| Product Name | RANK |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TNFRSF11A CD265 CD265 antigen Activator of NFKB EOF FEO mRANK NFKB activator ODFR OFE Osteoclast differentiation factor receptor PDB 2 Receptor activator of NF KB receptor activator of nuclear factor kappa B TNFRSF 11A TNFSF11 TRANCE R RANK receptor TNR11_HUMAN. |
| Product description | Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. |
| Immunogen | KLH conjugated synthetic peptide derived from human RANK 51-150/616 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg /test: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | TNFRSF11A CD265 CD265 antigen Activator of NFKB EOF FEO mRANK NFKB activator ODFR OFE Osteoclast differentiation factor receptor PDB 2 Receptor activator of NF KB receptor activator of nuclear factor kappa B TNFRSF 11A TNFSF11 TRANCE R RANK receptor TNR11_HUMAN. |
|---|---|
| Molecular Weight(MW) | 63-70 KDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q9Y6Q6 |
|---|
Application
Fig1: Sample: U251 Cell Lysate at 30 ug Primary: Anti- RANK (ER1915-70) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63-70 kD Observed band size: 63 kD
Application
Fig2: Blank control(blue):Jurkat cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-RANK antibody(ER1915-70), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Application
Fig3: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-RANK (ER1915-70) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63-70 kD Observed band size: 70 kD| Positive Control | Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg /test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
