Application
Fig1: Sample: heart (mouse) Lysate at 40 ug Primary: Anti- RBM20 (ER1915-73)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75kD Observed band size: 75 kDApplication
Fig2: Sample: K562(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti- RBM20 (ER1915-73) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 134 kD Observed band size: 130 kDApplication
Fig3: Blank control:A549. Primary Antibody (green line): Rabbit Anti-RBM20 antibody (ER1915-73) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | RBM20 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Probable RNA-binding protein 20 Rbm20 RBM20_HUMAN RNA-binding motif protein 20. |
| Product description | RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes involved in cardiac development. Regulates splicing of TTN (Titin). |
| Immunogen | KLH conjugated synthetic peptide derived from human RBM20 361-460/1227 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3ug/test: |
|
| Species Reactivity | HumanMouseRatDogCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Probable RNA-binding protein 20 Rbm20 RBM20_HUMAN RNA-binding motif protein 20. |
|---|---|
| Molecular Weight(MW) | 134 KDa |
| Cellular Localization | Nucleus (By similarity). |
| SwissProt ID | Q5T481 |
|---|
Application
Fig1: Sample: heart (mouse) Lysate at 40 ug Primary: Anti- RBM20 (ER1915-73)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75kD Observed band size: 75 kD
Application
Fig2: Sample: K562(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti- RBM20 (ER1915-73) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 134 kD Observed band size: 130 kD
Application
Fig3: Blank control:A549. Primary Antibody (green line): Rabbit Anti-RBM20 antibody (ER1915-73) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Expressed in the heart. |
|---|---|
| Application Notes | WB:1:500-2000: FC:3ug/test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
