Application
Fig1: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-RBM22 (ER1915-74) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 47 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RBM22) Polyclonal Antibody, Unconjugated (ER1915-74) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | RBM22 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cwc2 FLJ10290 fSAP47 Functional spliceosome associated protein 47 Pre mRNA splicing factor RBM22 Pre-mRNA-splicing factor RBM22 RBM 22 rbm22 RBM22_HUMAN RNA binding motif protein 22 RNA-binding motif protein 22 ZC3H16 Zinc finger CCCH domain containing protein 16 Zinc finger CCCH domain-containing protein 16. |
| Product description | Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses. |
| Immunogen | KLH conjugated synthetic peptide derived from human RBM22 101-300/420 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Cwc2 FLJ10290 fSAP47 Functional spliceosome associated protein 47 Pre mRNA splicing factor RBM22 Pre-mRNA-splicing factor RBM22 RBM 22 rbm22 RBM22_HUMAN RNA binding motif protein 22 RNA-binding motif protein 22 ZC3H16 Zinc finger CCCH domain containing protein 16 Zinc finger CCCH domain-containing protein 16. |
|---|---|
| Molecular Weight(MW) | 47 KDa |
| Cellular Localization | Nucleus. Cytoplasm. Mainly located in the nucleus. Translocated from the nucleus to the cytoplasm after heat shock cell treatment. May be shuttling between the nucleus and the cytosol. |
| SwissProt ID | Q9NW64 |
|---|
Application
Fig1: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-RBM22 (ER1915-74) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 47 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RBM22) Polyclonal Antibody, Unconjugated (ER1915-74) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
