Application
Fig1: Sample: heart (Mouse) Lysate at 40 ug Primary: Anti-REA (ER1915-76) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kDApplication
Fig2: Protein: spleen(mouse) lysate at 40ug; Primary: rabbit Anti-REA (ER1915-76) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 33 kD Observed band size: 35 kD| Product Name | REA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B cell receptor associated protein BAP37 B-cell associated protein B-cell receptor-associated protein BAP37 Bap 37 BAP Bap37 BCAP 37 BCAP37 D prohibitin D-prohibitin MGC117268 p22 PHB 2 PHB2 PHB2_HUMAN PNAS 141 PNAS141 Prohibitin 2 Prohibitin-2 Prohibitin2 Repressor of Estrogen Receptor Activity. |
| Product description | Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging. |
| Immunogen | KLH conjugated synthetic peptide derived from human REA 281-299/299 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | B cell receptor associated protein BAP37 B-cell associated protein B-cell receptor-associated protein BAP37 Bap 37 BAP Bap37 BCAP 37 BCAP37 D prohibitin D-prohibitin MGC117268 p22 PHB 2 PHB2 PHB2_HUMAN PNAS 141 PNAS141 Prohibitin 2 Prohibitin-2 Prohibitin2 Repressor of Estrogen Receptor Activity. |
|---|---|
| Molecular Weight(MW) | 33 KDa |
| Cellular Localization | Mitochondrion inner membrane. Cytoplasm. Nucleus. Also cytoplasmic and nuclear. |
| SwissProt ID | Q99623 |
|---|
Application
Fig1: Sample: heart (Mouse) Lysate at 40 ug Primary: Anti-REA (ER1915-76) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
Application
Fig2: Protein: spleen(mouse) lysate at 40ug; Primary: rabbit Anti-REA (ER1915-76) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 33 kD Observed band size: 35 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
