Application
Fig1: Blank control (Black line):Molt4 (Black). Primary Antibody (green line): Rabbit Anti-REC8 antibody (ER1915-77) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: HT29 Cell (Human) Lysate at 40 ug Primary: Anti-REC8 (ER1915-77) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 65 kDApplication
Fig3: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-REC8 (ER1915-77) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD| Product Name | REC8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cohesin Rec8p Meiotic recombination protein REC8 homolog Meiotic recombination protein REC8-like 1 rec8 REC8 homolog (yeast) REC8_HUMAN REC8L Rec8L1 Rec8p. |
| Product description | Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human REC8 22-120/547 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatDogPigHorseRabbitGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | Cohesin Rec8p Meiotic recombination protein REC8 homolog Meiotic recombination protein REC8-like 1 rec8 REC8 homolog (yeast) REC8_HUMAN REC8L Rec8L1 Rec8p. |
|---|---|
| Molecular Weight(MW) | 60 KDa |
| Cellular Localization | Nucleus. Chromosome. |
| SwissProt ID | O95072 |
|---|
Application
Fig1: Blank control (Black line):Molt4 (Black). Primary Antibody (green line): Rabbit Anti-REC8 antibody (ER1915-77) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: HT29 Cell (Human) Lysate at 40 ug Primary: Anti-REC8 (ER1915-77) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 65 kD
Application
Fig3: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-REC8 (ER1915-77) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD| Positive Control | Expressed in testis and thymus. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
