Application
Fig1: Sample: A431(human) cell Lysate at 40 ug Primary: Anti- repetin (ER1915-82) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 91kD Observed band size: 91 kDApplication
Fig2: Sample: Hela(human)cell Lysate at 40 ug Primary: Anti- repetin (ER1915-82) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 91kD Observed band size: 91 kD| Product Name | Repetin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Intermediate filament associated protein RPTN RPTN_HUMAN. |
| Product description | Involved in the cornified cell envelope formation. Multifunctional epidermal matrix protein. Reversibly binds calcium. |
| Immunogen | KLH conjugated synthetic peptide derived from human Repetin 41-140/784 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | Intermediate filament associated protein RPTN RPTN_HUMAN. |
|---|---|
| Molecular Weight(MW) | 91 KDa |
| Cellular Localization | Secreted; extracellular space; extracellular matrix. |
| SwissProt ID | Q6XPR3 |
|---|
Application
Fig1: Sample: A431(human) cell Lysate at 40 ug Primary: Anti- repetin (ER1915-82) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 91kD Observed band size: 91 kD
Application
Fig2: Sample: Hela(human)cell Lysate at 40 ug Primary: Anti- repetin (ER1915-82) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 91kD Observed band size: 91 kD| Positive Control | Repetin is a multifunctional epidermal matrix protein involved in the cornified cell envelope formation. It reversibly binds calcium. Expression is scattered in the normal epidermis but strong in the acrosyringium, the inner hair root sheat and in the filiform papilli of the tongue. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
