Application
Fig1: Sample: Cerebrum(Mouse) Cell Lysate at 40 ug Primary: Anti-RGMA (ER1915-84) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28/50 kD Observed band size: 50 kD| Product Name | RGMA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Repulsive guidance molecule A RGM domain family member A RGMA RGMA_HUMAN. |
| Product description | Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. |
| Immunogen | KLH conjugated synthetic peptide derived from human Repulsive Guidance Molecule A 301-400/1450 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatPigCow |
| Concentration | 1mg/ml |
| Alternative Names | Repulsive guidance molecule A RGM domain family member A RGMA RGMA_HUMAN. |
|---|---|
| Molecular Weight(MW) | 28/50 KDa |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor |
| SwissProt ID | Q96B86 |
|---|
Application
Fig1: Sample: Cerebrum(Mouse) Cell Lysate at 40 ug Primary: Anti-RGMA (ER1915-84) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28/50 kD Observed band size: 50 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
