Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RIP2) Polyclonal Antibody, Unconjugated (ER1915-87) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-RIP2 (ER1915-87) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD| Product Name | RIP2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Receptor interacting protein 2 Receptor interacting serine threonine kinase 2 Receptor interacting serine/threonine protein kinase 2 RICK RIP 2 RIP LIKE INTERACTING CLARP KINASE RIPK 2 RIPK2 TNFRSF CARD 3 CARD carrying kinase CARD CONTAINING ICE ASSOCIATED KINASE CARD containing IL 1 beta ICE kinase CARD containing IL1 beta ICE kinase CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase CARD containing interleukin 1 beta converting enzyme associated kinase CARD3 CARDIAK CCK CLARP kinase GIG 30 GIG30 Growth inhibiting gene 30 Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase. |
| Product description | Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. |
| Immunogen | KLH conjugated synthetic peptide derived from human RIP2 401-540/540 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | Receptor interacting protein 2 Receptor interacting serine threonine kinase 2 Receptor interacting serine/threonine protein kinase 2 RICK RIP 2 RIP LIKE INTERACTING CLARP KINASE RIPK 2 RIPK2 TNFRSF CARD 3 CARD carrying kinase CARD CONTAINING ICE ASSOCIATED KINASE CARD containing IL 1 beta ICE kinase CARD containing IL1 beta ICE kinase CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase CARD containing interleukin 1 beta converting enzyme associated kinase CARD3 CARDIAK CCK CLARP kinase GIG 30 GIG30 Growth inhibiting gene 30 Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase. |
|---|---|
| Molecular Weight(MW) | 60 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | O43353 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RIP2) Polyclonal Antibody, Unconjugated (ER1915-87) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-RIP2 (ER1915-87) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD| Positive Control | Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
