Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated (ER1915-93) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated (ER1915-93) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated (ER1915-93) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug 293T(Human) Cell Lysate at 30 ug Ovary (Mouse) Lysate at 40 ug Primary: Anti- RNF128 (ER1915-93) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 62 kDApplication
Fig5: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti- RNF128 (ER1915-93) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 61kD| Product Name | RNF128 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin protein ligase RNF128 E3 ubiquitin-protein ligase RNF128 FLJ23516 Gene related to anergy in lymphocytes protein GRAIL ring finger protein 128 RN128_HUMAN RNF128. |
| Product description | E3 ubiquitin-protein ligase that catalyzes polyubiquitin chains. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription and this activity is likely to be mediated by E3 ligase activity. Plays an important role in the induction of the anergic phenotype. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals. |
| Immunogen | KLH conjugated synthetic peptide derived from human RNF128 151-260/428 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:50-200: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | E3 ubiquitin protein ligase RNF128 E3 ubiquitin-protein ligase RNF128 FLJ23516 Gene related to anergy in lymphocytes protein GRAIL ring finger protein 128 RN128_HUMAN RNF128. |
|---|---|
| Molecular Weight(MW) | 43 KDa |
| Cellular Localization | Endomembrane system; Single-pass membrane protein. Cytoplasm, perinuclear region. Note=Localized in an asymmetric perinuclear punctuate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7A. |
| SwissProt ID | Q8TEB7 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated (ER1915-93) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated (ER1915-93) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated (ER1915-93) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug 293T(Human) Cell Lysate at 30 ug Ovary (Mouse) Lysate at 40 ug Primary: Anti- RNF128 (ER1915-93) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 62 kD
Application
Fig5: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti- RNF128 (ER1915-93) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 61kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:50-200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
