Application
Fig1: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti- RNF3 (ER1915-99) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28kD Observed band size: 28kD| Product Name | RNF3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DONG1 PCGF3 polycomb group ring finger 3 Ring finger protein 3 RNF3 RNF3A PCGF3_HUMAN. |
| Product description | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. |
| Immunogen | KLH conjugated synthetic peptide derived from human PCGF3/RNF3 141-242/242 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | DONG1 PCGF3 polycomb group ring finger 3 Ring finger protein 3 RNF3 RNF3A PCGF3_HUMAN. |
|---|---|
| Molecular Weight(MW) | 28 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q3KNV8 |
|---|
Application
Fig1: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti- RNF3 (ER1915-99) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28kD Observed band size: 28kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
