Application
Fig1: Sample: Large intestine (Mouse) Lysate at 40 ug Primary: Anti-RNF35 (ER1916-01) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD| Product Name | RNF35 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RING finger protein 35 Gm319 Gm35 Gm865 ring finger protein 40 Ring finger RNF35 TRIM40 Tripartite motif containing 40 Tripartite motif containing protein 40 Tripartite motif protein 40 TRI40_HUMAN. |
| Product description | May function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway. Promotes neddylation of IKBKG/NEMO, stabilizing NFKBIA, and inhibiting of NF-kappaB nuclear translocation and activity. |
| Immunogen | KLH conjugated synthetic peptide derived from human RNF35 81-180/258 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatPigCow |
| Concentration | 1mg/ml |
| Alternative Names | RING finger protein 35 Gm319 Gm35 Gm865 ring finger protein 40 Ring finger RNF35 TRIM40 Tripartite motif containing 40 Tripartite motif containing protein 40 Tripartite motif protein 40 TRI40_HUMAN. |
|---|---|
| Molecular Weight(MW) | 29 KDa |
| SwissProt ID | Q6P9F5 |
|---|
Application
Fig1: Sample: Large intestine (Mouse) Lysate at 40 ug Primary: Anti-RNF35 (ER1916-01) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD| Positive Control | Highly expressed in normal gastrointestinal epithelia but that is down-regulated in gastrointestinal carcinomas and chronic inflammatory lesions of the gastrointestinal tract. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
