Application
Fig1: Protein: Raji lysate at 40ug; Primary: rabbit Anti-RNF39 (ER1916-02) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 38/45 kD Observed band size: 38 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF39) Polyclonal Antibody, Unconjugated (ER1916-02) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | RNF39 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HZF HZFW HZFW1 LIRF Protein HZFw RING finger protein 39 Rnf39 RNF39_HUMAN. |
| Product description | May play a role in prolonged long term-potentiation (LTP) maintenance. |
| Immunogen | KLH conjugated synthetic peptide derived from human RNF39 182-230/420 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogMonkey |
| Concentration | 1mg/ml |
| Alternative Names | HZF HZFW HZFW1 LIRF Protein HZFw RING finger protein 39 Rnf39 RNF39_HUMAN. |
|---|---|
| Molecular Weight(MW) | 38/45 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q9H2S5 |
|---|
Application
Fig1: Protein: Raji lysate at 40ug; Primary: rabbit Anti-RNF39 (ER1916-02) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 38/45 kD Observed band size: 38 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF39) Polyclonal Antibody, Unconjugated (ER1916-02) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Expressed in testis. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
