Application
Fig1: Sample: 293T(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti-RNF70 (ER1916-05) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 71 kD Observed band size: 71 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF70) Polyclonal Antibody, Unconjugated (ER1916-05) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | RNF70 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase Praja 1 E3 ubiquitin-protein ligase Praja-1 PJA1 PJA1_HUMAN PRAJA 1 Praja ring finger 1 Praja1 Ring Finger Protein 70. |
| Product description | Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting. |
| Immunogen | KLH conjugated synthetic peptide derived from human RNF70 541-653/643 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | E3 ubiquitin-protein ligase Praja 1 E3 ubiquitin-protein ligase Praja-1 PJA1 PJA1_HUMAN PRAJA 1 Praja ring finger 1 Praja1 Ring Finger Protein 70. |
|---|---|
| Molecular Weight(MW) | 71 KDa |
| SwissProt ID | Q8NG27 |
|---|
Application
Fig1: Sample: 293T(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti-RNF70 (ER1916-05) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 71 kD Observed band size: 71 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF70) Polyclonal Antibody, Unconjugated (ER1916-05) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Expressed in various regions of the brain including the cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Highest levels in the cerebral cortex. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
