Application
Fig1: Sample: A431(human) Cell Lysate at 40 ug Primary: Anti-ROR alpha (ER1916-07) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kDApplication
Fig2: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-ROR alpha (ER1916-07) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kDApplication
Fig3: Sample: Jurkat (Human) Cell Lysate at 40 ug Primary: Anti-ROR alpha(ER1916-07)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 59kD Observed band size: 59 kD| Product Name | ROR alpha |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NR1F1 Nuclear receptor ROR-alpha Nuclear receptor RZR-alpha Nuclear receptor subfamily 1 group F member 1 RAR-related orphan receptor A Retinoid-related orphan receptor-alpha Rora RORA_HUMAN RZR-ALPHA RZRA. |
| Product description | Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives. |
| Immunogen | KLH conjugated synthetic peptide derived from human ROR alpha 151-250/523 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | NR1F1 Nuclear receptor ROR-alpha Nuclear receptor RZR-alpha Nuclear receptor subfamily 1 group F member 1 RAR-related orphan receptor A Retinoid-related orphan receptor-alpha Rora RORA_HUMAN RZR-ALPHA RZRA. |
|---|---|
| Molecular Weight(MW) | 59 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P35398 |
|---|
Application
Fig1: Sample: A431(human) Cell Lysate at 40 ug Primary: Anti-ROR alpha (ER1916-07) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD
Application
Fig2: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-ROR alpha (ER1916-07) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD
Application
Fig3: Sample: Jurkat (Human) Cell Lysate at 40 ug Primary: Anti-ROR alpha(ER1916-07)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 59kD Observed band size: 59 kD| Positive Control | Widely expressed in a number of tissues. Expressed in both regulatory T-cells (Treg) and effector T-cells (Teff). |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
